1PSO

The crystal structure of human pepsin and its complex with pepstatin

Experimental procedure

Source type	ROTATING ANODE
Detector technology	AREA DETECTOR
Collection date	1994-06-17
Detector	SAN DIEGO MULTIWIRE DETECTION SYSTEM
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	72.068, 150.972, 40.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.185
RMSD bond length	0.011
RMSD bond angle	2.750 *
Data reduction software	SAN (DIEGO SOFTWARE)
Refinement software	GROMOS

Data quality characteristics

	Overall
High resolution limit [Å]	1.970 *
Rmerge	0.054 *
Total number of observations	166144 *
Number of reflections	31184
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	pepsin	20 (mg/ml)
2	1	drop	pepstasin	5 (mM)
3	1	drop	methanol