1PRZ

Crystal structure of pseudouridine synthase RluD catalytic module

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-24
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	52.261, 76.362, 85.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 * - 1.800
Rwork	0.218
R-free	0.24300
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.065 *
Total number of observations	107285 *
Number of reflections	32444
<I/σ(I)>	12.4
Completeness [%]	99.4	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8 *	4 *	PEG 8000, KCL, Glycerol, pH 7., microbatch under oil, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	PEG8000	14 (%(w/v))
2	1	2		550 (mM)
3	1	2	glycerol	12 (%)
4	1	1	protein	14 (mg/ml)
5	1	1	Tris-HCl	20 (mM)	pH8.
6	1	1		200 (mM)
7	1	1	dithiothreitol	10 (mM)
8	1	1	glycerol	5 (%)