1PQ5
Trypsin at pH 5, 0.85 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8110 |
Spacegroup name | P 1 |
Unit cell lengths | 32.890, 36.958, 39.781 |
Unit cell angles | 102.84, 104.54, 102.39 |
Refinement procedure
Resolution | 30.000 - 0.830 * |
Rwork | 0.098 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 0.840 |
High resolution limit [Å] | 0.830 | 0.830 |
Rmerge | 0.033 * | |
Number of reflections | 146839 * | |
<I/σ(I)> | 34 | 7.22 |
Completeness [%] | 91.6 | 80.6 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | 293 | Rypniewski, W.R., (1993) Protein Eng., 6, 341. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12.5 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 0.7 (M) | |
3 | 1 | drop | citrate | 50 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
5 | 1 | reservoir | sodium citrate | 0.1 (M) |