1PQ1

Crystal structure of Bcl-xl/Bim

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	39.100, 47.340, 49.280
Unit cell angles	90.00, 110.10, 90.00

Refinement procedure

Resolution	20.000 * - 1.650
R-factor	0.219
Rwork	0.219
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	mouse Bcl-xl pdb entry 1PQ0
RMSD bond length	0.005 *
RMSD bond angle	1.110 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.750
High resolution limit [Å]	1.650	1.650
Rmerge	0.062	0.218
Number of reflections	19877	1765 *
<I/σ(I)>	8.6	2.7
Completeness [%]	97.2	90.9
Redundancy	4.8	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	NH4H2PO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	16 (%)
2	1	reservoir	MES	0.1 (M)	pH6.5
3	1	reservoir	1,4-dioxane	5 (%)
4	1	drop	protein	15 (mg/ml)