1PPY

Native precursor of pyruvoyl dependent Aspartate decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5482
Spacegroup name	P 61 2 2
Unit cell lengths	70.983, 70.983, 216.386
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.020 - 1.950
R-factor	0.15941
Rwork	0.158
R-free	0.20200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aw8
RMSD bond length	0.014 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000 *	2.010
High resolution limit [Å]	1.950	1.950
Rmerge	0.059 *	0.290 *
Number of reflections	24605 *
<I/σ(I)>	24.5	3.1
Completeness [%]	96.4	89.3
Redundancy	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	19 *	NH42SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-9 (mg/ml)
2	1	reservoir	ammonium sulfate	1.6 (M)