1PPR
PERIDININ-CHLOROPHYLL-PROTEIN OF AMPHIDINIUM CARTERAE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-16 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.426, 116.296, 67.025 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.20100 |
| Structure solution method | SIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.390 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.265 * |
| Number of reflections | 96045 | |
| Completeness [%] | 92.5 | 82.4 * |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 17 * | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 4-6 (%) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | triethylammoniumphosphate | 24 (mM) | |
| 6 | 1 | drop | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 8-12 (%) |
