1PP7
Crystal structure of the T. vaginalis Initiator binding protein bound to the ferredoxin Inr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.06 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.340, 41.430, 58.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.430 - 2.450 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | one of the IBD-alphaSCS complexes that is being deposited simultaneous with this deposition |
| RMSD bond length | 0.017 |
| RMSD bond angle | 21.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.430 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 7158 | |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 94.5 | 98.4 |
| Redundancy | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 298 | PEG 8000, MES pH 6.0, 0.1 M Zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | Zinc acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 8000 | ||
| 6 | 1 | 2 | Zinc acetate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | 0.1 (M) | pH6.0 | |
| 3 | 1 | reservoir | zinc acetate | 0.1 (M) |
