1POV
ROLE AND MECHANISM OF THE MATURATION CLEAVAGE OF VP0 IN POLIOVIRUS ASSEMBLY: STRUCTURE OF THE EMPTY CAPSID ASSEMBLY INTERMEDIATE AT 2.9 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 322.900, 358.000, 380.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.281 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.700 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.189 * |
| Total number of observations | 1885617 * |
| Number of reflections | 610509 |
| Completeness [%] | 61.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | capsid | 15 (mg/ml) | |
| 2 | 1 | 1 | 0.8 (M) | ||
| 3 | 1 | 1 | PIPES | 10 (mM) | |
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 1 | 1 (mM) | ||
| 6 | 1 | 2 | 0.05 (M) | ||
| 7 | 1 | 2 | PIPES | 10 (mM) | |
| 8 | 1 | 2 | 5 (mM) | ||
| 9 | 1 | 2 | 1 (mM) |
