1POB

CRYSTAL STRUCTURE OF COBRA-VENOM PHOSPHOLIPASE A2 IN A COMPLEX WITH A TRANSITION-STATE ANALOGUE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	34.600, 73.500, 181.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.000
R-factor	0.179
Rwork	0.179
RMSD bond length	0.014
RMSD bond angle	2.300
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.070 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		2 (mM)
3	1	drop		10 (mM)
4	1	drop	Tris-HCl	0.1 (M)
5	1	drop	ammonium sulfate	0.7 (M)
6	1	reservoir	ammonium sulfate	1.4 (M)
7	1	reservoir	Tris-HCl	0.1 (M)