1PNE
CRYSTALLIZATION AND STRUCTURE DETERMINATION OF BOVINE PROFILIN AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
Collection date | 1993 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.150, 34.590, 52.490 |
Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.165 |
Rwork | 0.165 |
RMSD bond length | 0.011 |
RMSD bond angle | 24.990 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.066 |
Total number of observations | 29297 * |
Number of reflections | 8239 |
Completeness [%] | 95.9 |
Redundancy | 3.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG400 | 1.5 (%) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
4 | 1 | drop | HEPES | 0.1 (M) | |
5 | 1 | drop | profilin | 14 (mg/ml) |