1PMY

REFINED CRYSTAL STRUCTURE OF PSEUDOAZURIN FROM METHYLOBACTERIUM EXTORQUENS AM1 AT 1.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	52.619, 63.280, 35.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.199
Rwork	0.199
RMSD bond length	0.024
RMSD bond angle	0.039 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.200 *	1.200 *
Rmerge	0.087 *	0.039 *
Total number of observations	94051 *
Number of reflections	25154 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	sodium azide	1 (mM)
4	1	drop	ammonium sulfate	40 (%sat)
5	1	reservoir	potassium phosphate	50 (mM)
6	1	reservoir	sodium azide	1 (mM)
7	1	reservoir	ammonium sulfate	67 (%sat)