1PM1
Crystal structure of nitrophorin 2 L122V/L132V mutant complex with imidazole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 39.310, 125.470, 33.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.640 - 1.100 |
R-factor | 0.1547 |
Rwork | 0.154 |
R-free | 0.17700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1euo |
RMSD bond length | 0.022 |
RMSD bond angle | 1.936 |
Data reduction software | XDISPLAYF |
Data scaling software | d*TREK |
Refinement software | REFMAC (5.1.22) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.700 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.077 | 0.270 |
Number of reflections | 62884 | |
<I/σ(I)> | 9.3 | 1 |
Completeness [%] | 91.0 | 81.2 |
Redundancy | 3.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |