1PL9
Crystal structure of KDO8P synthase in its binary complex with substrate analog Z-FPEP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-10 |
Detector | MACSCIENCE |
Wavelength(s) | 0.934 |
Spacegroup name | I 2 3 |
Unit cell lengths | 117.055, 117.055, 117.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.28 |
Rwork | 0.233 |
R-free | 0.32710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1D9E (a) |
RMSD bond length | 1.718 |
RMSD bond angle | 0.009 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.890 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.102 | 0.110 |
Number of reflections | 6309 | |
<I/σ(I)> | 15 | |
Completeness [%] | 96.2 | 96.7 |
Redundancy | 4.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | EG 4000, Glycrol, Tris-HCl , pH 7.4 , VAPOR DIFFUSION, HANGING DROP, temperature 294 K |