1PKY
PYRUVATE KINASE FROM E. COLI IN THE T-STATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Collection date | 1994-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.911, 129.577, 241.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.203 |
| R-free | 0.30500 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.088 |
| Total number of observations | 540887 * |
| Number of reflections | 77917 |
| Completeness [%] | 99.9 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 2 | 1 | reservoir | 10 (mM) | ||
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | MES/NaOH | 100 (mM) |
