1PKV
The N-terminal domain of riboflavin synthase in complex with riboflavin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8424 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.161, 104.383, 85.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.600 |
| R-factor | 0.17699 |
| Rwork | 0.174 |
| R-free | 0.23469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i8d |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.798 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.630 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 7131 | |
| Completeness [%] | 99.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 70 mM sodium potassium phosphate, pH 7.0, 100 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
