1PKO
Myelin Oligodendrocyte Glycoprotein (MOG)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0500 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.234, 50.234, 76.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| Rwork | 0.194 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.051 | 0.398 |
| Number of reflections | 20292 * | |
| <I/σ(I)> | 28.1 | 3.9 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 18 * | Tris/HCl, PEG8000, MgCl2, NaCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 18 * | Tris/HCl, PEG8000, MgCl2, NaCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 30 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.2 |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 22 (%) |
