1PKG
Structure of a c-Kit Kinase Product Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.667, 116.458, 60.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.22874 |
| Rwork | 0.225 |
| R-free | 0.31000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.610 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.900 | 2.900 * |
| Rmerge | 0.072 * | 0.424 * |
| Total number of observations | 49361 * | |
| Number of reflections | 14652 | |
| Completeness [%] | 97.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 * | 295 | PEG, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 6 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris | 25 (mM) | pH7.9 |
| 4 | 1 | drop | ATP | 2.5 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 18 (%) | |
| 7 | 1 | reservoir | MES | 0.1 (M) | pH7.1 |
