1PK8
Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-17 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.600, 78.400, 135.000 |
| Unit cell angles | 80.60, 76.90, 71.80 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aux ATPgammaS and Ca2+ removed |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.058 * | 0.373 * |
| Total number of observations | 522852 * | |
| Number of reflections | 144586 * | |
| <I/σ(I)> | 20.9 | 2.8 |
| Completeness [%] | 96.3 | 92 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 299 | PEGMME 5000, Tris, NaCl, Ca.ATP, EDTA, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | calcium ATP | 10 (mM) | |
| 3 | 1 | reservoir | Tris | 150 (mM) | pH7.5 |
| 4 | 1 | reservoir | PEG5000 MME | 15-20 (%(w/v)) | |
| 5 | 1 | reservoir | 300 (mM) |
