1PK5
Crystal structure of the orphan nuclear receptor LRH-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.775, 127.542, 53.213 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 24.550 - 2.400 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.23100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RXR ALPHA (PDB ID 1FBY) |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.570 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.550 |
| High resolution limit [Å] | 2.400 * | 2.400 |
| Rmerge | 0.067 | 0.169 |
| Number of reflections | 17954 * | |
| <I/σ(I)> | 24.4 | 5.7 |
| Completeness [%] | 98.2 * | 88.8 * |
| Redundancy | 6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.8 | 15 * | PEG 4000, glycerol, TRIS, isopropanol, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | glycerol | 15 (%) | |
| 3 | 1 | reservoir | PEG4000 | 21 (%) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.8 |
| 5 | 1 | reservoir | isopropanol | 5 (%) |
