1PJC
L-ALANINE DEHYDROGENASE COMPLEXED WITH NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-19 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 122.100, 122.100, 184.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.200 |
| R-free | 0.26000 |
| Structure solution method | ISOMORPHOUS TO NATIVE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.430 |
| Number of reflections | 188299 | |
| <I/σ(I)> | 13.1 | 1.6 |
| Completeness [%] | 92.0 | 94.1 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | PROTEIN CRYSTALLISED FROM 0.1M MES PH 6.7 6-10% PEG 20000, SOAKED IN 10MM NAD, pH 6.70 |
