1PJ7

Structure of dimethylglycine oxidase of Arthrobacter globiformis in complex with folinic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-08-01
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	C 2 2 2
Unit cell lengths	70.621, 223.740, 119.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.16253
Rwork	0.159
R-free	0.22200 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1pj6
RMSD bond length	0.021
RMSD bond angle	1.837 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.073	0.344 *
Number of reflections	46564
<I/σ(I)>	6.2
Completeness [%]	88.3	84.5
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	15% PEG 2000MME, 0.2M MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG5000 MME	15 (%)
3	1	reservoir		0.2 (M)	pH7.5
4	1	reservoir	HEPES	0.1 (M)