1PII

THREE-DIMENSIONAL STRUCTURE OF THE BIFUNCTIONAL ENZYME PHOSPHORIBOSYLANTHRANILATE ISOMERASE: INDOLEGLYCEROLPHOSPHATE SYNTHASE FROM ESCHERICHIA COLI REFINED AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	104.700, 104.700, 68.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.173
RMSD bond length	0.010
RMSD bond angle	3.200
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.133 *
Total number of observations	130573 *
Number of reflections	45216 *
Completeness [%]	92.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *		taken from White, J.L. et al (1982). FEBS Lett., 148, 87-90. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5-10 (mg/ml)
2	1	drop	ammonium sulfate	0.8 (M)
3	1	drop	potassium phosphate	50 (mM)
4	1	drop	EDTA	5 (mM)
5	1	drop	DTE	1-2 (mM)
6	1	reservoir	ammonium sulfate	1.6 (M)
7	1	reservoir	potassium phosphate	50 (mM)