1PIG

PIG PANCREATIC ALPHA-AMYLASE COMPLEXED WITH THE OLIGOSACCHARIDE V-1532

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	275
Detector technology	IMAGE PLATE
Collection date	1995-07-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	70.500, 114.800, 118.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.178
Rwork	0.178
R-free	0.21000
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1pif
RMSD bond length	0.007
RMSD bond angle	25.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.500	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.123
Number of reflections	47260
Completeness [%]	93.7	97.8
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6		VAPOR DIFFUSION; 10 MICROLITER OF PROTEIN (15 MG/ML IN 0.1 M AMMONIUM CACODYLATE, PH 10.0 0.001 M V-1532) WERE STEPWISE CONCENTRATED OVER 0.1, 0.15, AND 0.25 M AMMONIUM CACODYLATE, PH 6.0., vapor diffusion

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	cacodylate	0.01 (M)
3	1	drop		0.002 (M)