1PHW
Crystal structure of KDO8P synthase in its binary complex with substrate analog 1-deoxy-A5P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 118.258, 118.258, 118.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.360 |
| R-factor | 0.2604 |
| Rwork | 0.237 |
| R-free | 0.31270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1D9E (a) |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.047 | 0.472 |
| Number of reflections | 11064 | |
| <I/σ(I)> | 17.6 | 5.5 |
| Completeness [%] | 96.3 | 97.9 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | PEG 6000, Glycrol, Tris-HCl , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 294 K |
