1PHS

THE THREE-DIMENSIONAL STRUCTURE OF THE SEED STORAGE PROTEIN PHASEOLIN AT 3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	126.800, 134.500, 161.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.225 *
Rwork	0.225
RMSD bond length	0.015
RMSD bond angle	3.730
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.102 *
Number of reflections	41688 *
Completeness [%]	74.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			taken from Suzuki, E. et al (1983). J. Biol. Chem., 258, 2634-2636. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	50 (%sat)
3	1	reservoir	ammonium sulfate	70 (%sat)	or 23% ethanol