1PFZ
PROPLASMEPSIN II FROM PLASMODIUM FALCIPARUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.007, 133.223, 114.234 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.077 | 0.396 |
| Number of reflections | 114943 | |
| <I/σ(I)> | 7.8 | 3 |
| Completeness [%] | 96.9 | 91.5 |
| Redundancy | 5.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 105 * | 2 microlitter of protein splution was mixed with 7 microlitter of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 25 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.18 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 5 | 1 | reservoir | beta-octylglucoside | 0.1 (%) |
