1PFV
METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH DIFLUOROMETHIONINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.573, 45.221, 86.068 |
| Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
| Resolution | 74.000 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.20400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qqt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.180 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 * | 0.190 * |
| Number of reflections | 63973 * | |
| <I/σ(I)> | 9.4 | 3.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 280 | Mechulam, Y., (1999) J. Mol. Biol., 294, 1287. * |
