1PF8

Crystal Structure of Human Cyclin-Dependent Kinase 2 Complexed with a Nucleoside Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-14
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.753, 72.306, 71.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500 *
R-factor	0.208
Rwork	0.208
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ckp
RMSD bond length	0.007
RMSD bond angle	1.259
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.590
High resolution limit [Å]	2.500 *	2.510
Rmerge	0.066	0.223
Total number of observations	66150 *
Number of reflections	9694 *
Completeness [%]	99.6	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	4 *	HEPES, EDTA, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	50 (mM)	pH7.4
3	1	drop	EDTA	1 (mM)
4	1	reservoir	HEPES	200 (mM)	pH7.4