1PF7
CRYSTAL STRUCTURE OF HUMAN PNP COMPLEXED WITH IMMUCILLIN H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Collection date | 2003-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.390, 139.390, 161.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.600 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m73 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.893 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.531 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 16763 | |
| <I/σ(I)> | 6.6 | 1.9 |
| Completeness [%] | 90.1 | 93.4 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 0.05M CITRATE BUFFER, 19% AMMONIUM SULPHATE, pH 5.30 |
