1PF5
Structural Genomics, Protein YJGH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-01 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 127.737, 127.737, 127.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.160 - 2.500 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.872 |
| Number of reflections | 11955 | |
| <I/σ(I)> | 22.04 | 1.68 |
| Completeness [%] | 98.2 | 99.5 |
| Redundancy | 8.31 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Lithium sulphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
