1PCF

HUMAN TRANSCRIPTIONAL COACTIVATOR PC4 C-TERMINAL DOMAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	41.283, 67.814, 67.170
Unit cell angles	87.69, 84.37, 85.79

Refinement procedure

Resolution	20.000 - 1.740
R-factor	0.197 *
Rwork	0.197
R-free	0.23300
Structure solution method	SIRAS
RMSD bond length	0.013
RMSD bond angle	0.028
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CCP4

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.780
High resolution limit [Å]	1.740	1.740
Rmerge	0.060	0.229
Total number of observations	177425 *
Number of reflections	69529
<I/σ(I)>	11.6	2.6
Completeness [%]	93.4	83.4
Redundancy	2.6	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *		PROTEIN WAS CRYSTALLIZED FROM 25% MPD, 200 MM NACL AND 100 MM NAAC BUFFER (PH 4.6)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	MPD	25 (%(v/v))
2	1	2		0.2 (M)
3	1	2	sodium acetate	0.1 (M)
4	1	1	protein	7-10 (mg/ml)
5	1	1	HEPES	10 (mM)
6	1	1		0.2 (M)