1PC5
Crystal Structure of the P50G Mutant of Ferredoxin I at 1.8 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5408 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.166, 55.166, 91.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.25300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 13643 | |
| Completeness [%] | 92.2 | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 2 * | Shen, B., (1993) J.Biol.Chem., 268, 25928., Stout, C.D., (1979) J.Biol.Chem., 254, 3598. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 0.5 (M) | |
| 3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.5 (M) |
