1PBK

HOMOLOGOUS DOMAIN OF HUMAN FKBP25

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-12-28
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 41 21 2
Unit cell lengths	86.000, 86.000, 55.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.191 *
Rwork	0.185
R-free	0.24400 *
RMSD bond length	0.011
RMSD bond angle	2.880 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.065	0.285 *
Total number of observations	24730 *
Number of reflections	7520 *
Completeness [%]	98.1 *	99.4 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	30-35 (%(w/v))
2	1	reservoir	HEPES	100 (mM)