1PAR
DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.670, 56.730, 53.800 |
| Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
| Resolution | 21.000 - 2.600 |
| R-factor | 0.225 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.510 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.114 * |
| Total number of observations | 161619 * |
| Number of reflections | 11253 |
| Completeness [%] | 95.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | pH 7.40, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 3 | 1 | 1 | NA AZIDE | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Arc tetramer | 0.58 (mM) | |
| 2 | 1 | drop | DNA duplex | 0.93 (mM) | |
| 3 | 1 | drop | Bis-Tris-propane-Cl | 30 (mM) | pH7.4 |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | reservoir | PEG400 | 15 (%) | |
| 6 | 1 | reservoir | 100 (mM) |
