1PAQ

CRYSTAL STRUCTURE OF THE CATALYTIC FRAGMENT OF EUKARYOTIC INITIATION FACTOR 2B EPSILON

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-10
Detector	MARRESEARCH
Wavelength(s)	0.9821, 0.9824, 0.9121
Spacegroup name	I 4 2 2
Unit cell lengths	106.340, 106.340, 91.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.2439
Rwork	0.243
R-free	0.27200 *
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.050 *	0.278 *
Number of reflections	22162
Completeness [%]	99.2	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *	5 *	PEG 2000MME, ammonium acetate, Tris-HCl, pH 5.75, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.4
2	1	drop	dithiothreitol	5 (mM)
3	1	drop	PMSF	1 (mM)
4	1	drop	EDTA	0.5 (mM)
5	1	drop		100 (mM)
6	1	drop	protein	5-8 (mg/ml)
7	1	reservoir	ammonium acetate	0.2 (M)
8	1	reservoir	PEG2000 MME	23-25 (%)
9	1	reservoir	sodium citrate	100 (mM)	pH7.57