1PA6
Crystal structure of the OXYTRICHA nova telomere end-binding protein complexed with noncognate ssDNA GGGGTTTTGAGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.110, 93.110, 422.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.790 - 2.450 |
| R-factor | 0.264 |
| Rwork | 0.223 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.790 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 31008 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Horvath, M.P., (1998) Cell., 95, 963. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ethylene glycol | 30 (%) | |
| 2 | 1 | drop | PEG4000 | 1-6 (%) | |
| 3 | 1 | drop | MES | 40 (mM) | pH5.5-7.0 |
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | drop | dithiothreitol | 2 (mM) | |
| 6 | 1 | reservoir | 50 (mM) |
