1PA2
ARABIDOPSIS THALIANA PEROXIDASE A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.156, 74.597, 80.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 1.450 |
| R-factor | 0.1398 * |
| R-free | 0.18910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.058 * | 0.243 * |
| Total number of observations | 280003 * | |
| Number of reflections | 49745 | 5403 * |
| <I/σ(I)> | 19.1 | 8.4 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.98 (mg/ml) | |
| 2 | 1 | drop | magnesium acetate | 0.1 (M) | |
| 3 | 1 | drop | cacodylate | 0.5 (M) | |
| 4 | 1 | drop | PEG8000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 6 | 1 | reservoir | cacodylate | 0.1 (M) | |
| 7 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |
