1P9Y

Ribosome binding of E. coli Trigger Factor mutant F44L.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-13
Detector	MARRESEARCH
Wavelength(s)	1.12
Spacegroup name	H 3 2
Unit cell lengths	71.901, 71.901, 257.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 * - 2.150
R-factor	0.2
Rwork	0.200
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oms
RMSD bond length	0.007
RMSD bond angle	22.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.078	0.201
Number of reflections	14381
<I/σ(I)>	25.2	6.5
Completeness [%]	99.7	96.7
Redundancy	11.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	6 *	PEG 2000 MME, AMMONIUM SULFATE, SODIUM ACETATE, TRIS, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 281K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.5 (mg/ml)
2	1	reservoir	ammonium sulfate	0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)	pH5.0
4	1	reservoir	PEG400	5 (%)
5	1	reservoir	glycerol	5 (%)
6	1	reservoir	PEG2000 MME	31 (%)