1P9R
Crystal Structure of Vibrio cholerae putative NTPase EpsE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9748 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 103.611, 103.611, 166.261 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.21653 |
Rwork | 0.214 |
R-free | 0.26500 * |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.374 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE (V. 2.02) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.102 * | |
Total number of observations | 241296 * | |
Number of reflections | 18982 | |
<I/σ(I)> | 18.6 | 4.3 |
Completeness [%] | 100.0 | 65.3 * |
Redundancy | 12.71 * | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 * | 293 | PEG 200,3-morpholinopropanesulfonate, ammonium acetate, AMP-PNP, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | TEA | 0.1 (M) | |
3 | 1 | drop | 0.5 (M) | ||
4 | 1 | drop | glycerol | 10 (%) | |
5 | 1 | drop | TCEP | 1 (mM) | |
6 | 1 | drop | EDTA | 1 (mM) | |
7 | 1 | reservoir | PEG5000 MME | 12-18 (%(v/v)) | |
8 | 1 | reservoir | ammonium sulfate | 0.15-0.20 (M) | |
9 | 1 | reservoir | MES | 0.1 (M) | pH6.3 |