1P9N

Crystal structure of Escherichia coli MobB.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97936
Spacegroup name	P 64 2 2
Unit cell lengths	239.001, 239.001, 49.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 2.800
R-factor	0.275
Rwork	0.275
R-free	0.30800
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	24.300 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.800 *	2.610
Rmerge	0.183 *	0.716 *
Total number of observations	241509 *
Number of reflections	20053 *
Completeness [%]	98.5 *	87.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	295	1.68 M ammonium sulphate, 50 mM Malonic acid, 15 mM cetyl ammonium bromide, 10 mM MgCl2, 1 mM B-mercaptoethanol., pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.0 (mg/ml)
10	1	reservoir	beta-mercaptoethanol	1 (mM)
2	1	drop	Tris	50 (mM)	pH7.5
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	drop	glycerol	5 (%(v/v))
6	1	reservoir	ammonium sulfate	1.68 (M)
7	1	reservoir	malonic acid	0.5 (M)	pH4.5
8	1	reservoir	acetyl ammonium bromide	15 (mM)
9	1	reservoir		10 (mM)