1P9A
Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 1.7 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0642 |
| Spacegroup name | P 43 |
| Unit cell lengths | 51.734, 51.734, 114.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2.7 Angstrom P21/GPIb-alpha MAD structure-to be published |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 160737 | |
| <I/σ(I)> | 19.8 | 5.3 |
| Completeness [%] | 95.4 | 73.6 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | PEG 6000, Sodium Nitrate, Sodium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 2.5 (mM) | |
| 2 | 1 | drop | 50 (mM) | pH7.4 | |
| 3 | 1 | drop | protein | 15-20 (mg/ml) | |
| 4 | 1 | reservoir | PEG6000 | 12 (%(w/w)) | |
| 5 | 1 | reservoir | sodium nitrate | 200 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.6 |
