1P91

Crystal Structure of RlmA(I) enzyme: 23S rRNA n1-G745 methyltransferase (NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER19)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	105
Detector technology	CCD
Collection date	2002-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97889, 0.97874, 0.9500
Spacegroup name	I 2 2 2
Unit cell lengths	107.187, 122.279, 143.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.248
Rwork	0.248
R-free	0.29600
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.270 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.106
Total number of observations	88268 *
Number of reflections	21876
<I/σ(I)>	11
Completeness [%]	93.0	80.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	300	Ammonium sulfate, Lithium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 300K
1	Vapor diffusion, hanging drop *	7.5 *	300	Ammonium sulfate, Lithium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.5
3	1	drop	SAM	5 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	ammonium sulfate	0.5 (M)
6	1	reservoir	lithium sulfate	1.0 (M)
7	1	reservoir	sodium citrate	0.1 (M)	pH6.5