1P8X

The Calcium-Activated C-terminal half of gelsolin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-13
Detector	ADSC QUANTUM 4
Wavelength(s)	1.068
Spacegroup name	P 21 21 21
Unit cell lengths	84.750, 90.110, 156.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.205
Rwork	0.205
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The gelsolin (G4-G6) portion of PDB Entry 1H1V
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.054	0.130
Total number of observations	512059 *
Number of reflections	81129 *	11619 *
<I/σ(I)>	6.6	6
Completeness [%]	99.2	98.7
Redundancy	6.3	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	15% (w/v) PEG 8000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	15 (%(w/v))
3	1	reservoir	Tris-HCl	100 (mM)	pH7.5