1P84
HDBT inhibited Yeast Cytochrome bc1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2000-12-04 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.998, 165.091, 147.525 |
| Unit cell angles | 90.00, 117.33, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| Rwork | 0.228 |
| R-free | 0.25200 |
| Structure solution method | Positional and B-factor refinement |
| Starting model (for MR) | 1kb9 protein only |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.298 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 | 0.373 * |
| Total number of observations | 372220 * | |
| Number of reflections | 149103 | 9128 * |
| <I/σ(I)> | 13.4 | 1.2 |
| Completeness [%] | 92.4 | 84.6 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 4 * | PEG4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 5 (%) | pH7.5 |
| 3 | 1 | reservoir | n-undecyl-beta-D-maltopyranoside | 0.05 (%) | |
| 4 | 1 | reservoir | HHDBT | 10000 (nM) | pH8.0 |
| 5 | 1 | reservoir | Tris-HCl |
