1P7P
Methionyl-tRNA synthetase from Escherichia coli complexed with methionine phosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.360, 45.000, 85.950 |
| Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qqt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.870 |
| High resolution limit [Å] | 1.800 * | 1.780 |
| Rmerge | 0.059 * | 0.280 * |
| Number of reflections | 52574 * | |
| Completeness [%] | 97.8 | 93.4 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 280 | Mechulam, Y., (1999) J. Mol. Biol., 294, 1287. * |
