1P5Z
Structure of human dCK complexed with cytarabine and ADP-MG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-11-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.720, 80.720, 94.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.1754 |
Rwork | 0.173 |
R-free | 0.19700 * |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.770 |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.052 * | 0.511 * |
Total number of observations | 487680 * | |
Number of reflections | 41303 | |
<I/σ(I)> | 23.1 | 4.9 |
Completeness [%] | 99.0 * | 99.6 * |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | 20%(w/v)PEG1K, 100mM magnesium acetate, 100mM TRIS, 5mM araC, 5mM ADP, 5mM MgCl2, 5mM DTT, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 12 (mg/ml) | |
2 | 1 | drop | 5 (mM) | ||
3 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | AraC | 100000 (nM) | |
6 | 1 | reservoir | trisodium citrate dihydrate | 0.95-1.0 (M) | |
7 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |