1P5V

X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	36.000, 69.230, 69.110
Unit cell angles	90.00, 92.96, 90.00

Refinement procedure

Resolution	20.000 - 1.800 *
R-factor	0.201
Rwork	0.198
R-free	0.24100 *
Structure solution method	MAD
RMSD bond length	0.027
RMSD bond angle	2.200 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.077	0.791
Number of reflections	34122
<I/σ(I)>	20.9	1.6
Completeness [%]	90.5	69.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. *
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. *
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. *
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Zavialov, A., (2003) Acta Crystallogr.,Sect.D, 59, 359. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	PEG4000	20-30 (%)
3	1	reservoir	PEG8000	20-30 (%)