1P5C
Circular permutation of Helix A in T4 lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 170 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.758, 67.763, 94.057 |
Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lzm |
RMSD bond length | 0.012 |
RMSD bond angle | 13.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 22893 | |
<I/σ(I)> | 6.7 | |
Completeness [%] | 97.4 | 99.3 |
Redundancy | 2.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 30% PEG 3400, 50mM Phosphate buffer, 5% isopropanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |