1P59
Structure of a non-covalent Endonuclease III-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.321, 105.896, 41.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1orn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.100 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.056 | 0.271 |
| Number of reflections | 17421 | |
| <I/σ(I)> | 19.6 | 3.7 |
| Completeness [%] | 89.0 | 83.1 |
| Redundancy | 3.3 * | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 8000, TRIS, BME, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | TRIS | ||
| 3 | 1 | 1 | BME | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | TRIS | ||
| 6 | 1 | 2 | BME |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 2 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
| 3 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) |
