1P4P
Outer Surface Protein B of B. burgdorferi: crystal structure of the C-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979, 1.073 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 30.800, 51.660, 158.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.211 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.206 |
| Number of reflections | 8090 | |
| <I/σ(I)> | 34.1 | 12.6 |
| Completeness [%] | 89.8 | 77.9 |
| Redundancy | 23.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | PEG550MME, Bicine, sodium bromide, sodium citrate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
